منابع مشابه
Transcription initiation site selection and abortive initiation cycling of phage SP6 RNA polymerase.
Effects of mutations around the phage SP6 transcription initiation site on SP6 RNA polymerase's selection of initiation site were studied. In the in vitro transcription reactions, the limiting concentration of a ribonucleotide causes the SP6 RNA polymerase to stall long enough only at the positions of the limited nucleotide and dissociate from the elongation complex. As a result, a series of RN...
متن کاملAbortive initiation and productive initiation by RNA polymerase involve DNA scrunching.
Using single-molecule DNA nanomanipulation, we show that abortive initiation involves DNA "scrunching"--in which RNA polymerase (RNAP) remains stationary and unwinds and pulls downstream DNA into itself--and that scrunching requires RNA synthesis and depends on RNA length. We show further that promoter escape involves scrunching, and that scrunching occurs in most or all instances of promoter e...
متن کاملTiny abortive initiation transcripts exert antitermination activity on an RNA hairpin-dependent intrinsic terminator
No biological function has been identified for tiny RNA transcripts that are abortively and repetitiously released from initiation complexes of RNA polymerase in vitro and in vivo to date. In this study, we show that abortive initiation affects termination in transcription of bacteriophage T7 gene 10. Specifically, abortive transcripts produced from promoter phi 10 exert trans-acting antitermin...
متن کاملAbortive Initiation as a Bottleneck for Transcription in the Early Drosophila Embryo
Gene transcription is a critical step in gene expression. The currently accepted physical model of transcription of MacDonald, Gibbs & Pipkin (1969) predicts the existence of a physical limit on the maximal rate of transcription, which does not depend on the transcribed gene. This limit appears as a result of polymerase “traffic jams” forming in the bulk of the 1D DNA chain at high polymerase c...
متن کاملThe Abortive Treatment of Gonorrhœa
To the Editor of The Indian Medical Gazette. Sir,?After reading through the extract from the Practitioner on the abortive treatment of gonorrhoea in the December 1921 issue of the Indian Medical Gazette, I decided to try it on the next suitable case I got. A Parsi, age 24, came to me on the 13th January, 1922, with a history that he had been exposed to infection on the 10th, and that the discha...
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ژورنال
عنوان ژورنال: Methods
سال: 2009
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2008.10.010